Figure 7

IVM medium supplementation with TAB cocktail does not improve the developmental competence of MGO-challenged DOs. DOs were cultured in IVM medium with indicated concentrations of MGO (20, 40, 75 and 150 µM) for 16–18 h and then IVF was carried out on matured DOs and then cultured in G-TL medium for 4.5 days; (A) Maturation rate/ GV, (B) 2PN formation rate/ GV (C) Blastocyst rate/ GV; DOs were cultured in IVM medium in presence of MGO (40 or 75 µM) and/or TAB (Taurine: 10 mM; ALA: 10µM; B6: 10 µM) for 16–18 h and then IVF was carried out on matured DOs and then cultured in G-TL medium for 4.5 days; (D) Maturation rate/ GV, (E) 2PN formation rate/ GV (F) Blastocyst rate/ GV; At least 50 DOs were cultured in each replicate of each treatment group. Five replicates were included. Data are presented as mean ± SEM. Different letters (a, b, and c) indicate significant differences between the treatment groups at P < 0.05. Statistical differences between groups were assessed using one-way ANOVA with LSD post-hoc test.