Figure 1
Light microscopy imaging (magnification ×4) of two exemplary organoid cultures (a,b) after stimulation with increasing TNFα concentrations (0, 0.1, 1, 10 and 100 ng/ml). Images were taken immediately before processing for flow cytometry after 72 h of proinflammatory stimulation. Without cytokine influence, enteroids present complex budding structures without accelerative debris. With increasing concentrations of TNFα, the enteroids present visible morphological changes with rounded buds and cell detritus inside the lumen as a sign for cell destruction, especially following concentrations 10 ng/ml and 100 ng/ml.
