Table 1 The sgRNAs in this study.
Primer | sgRNA Sequence (5′–3′) |
|---|---|
MYH6-1 | ATGCTTTAATGGCAGAGTTCGGG |
MYH6-2 | GCTCTTTGTCAGATTTCCTCAGG |
MYH6-3 | CAAACGCAGAGCCAAATAAGGG |
MYH6-4 | GGTTTATCTGCAGTTAAATGTGG |
MYH6-5 | AGTTGAGTTTCAAAGAGGGGGGG |
RASD1-1 | GGTGGCTTCGGAGCCTCTGAAGG |
RASD1-2 | TCCCGGCGAAAAACTGCTACCGG |
RASD1-3 | GCAGTACACGCCGACCATTGAGG |
RASD1-4 | GTCCTCTCAGTACACTTTCCTGG |
RASD1-5 | AAAAATAGATAGACTAATGTAGG |
sgRNA-R | AAAAAAAGCACCGACTCGGTGCCAC |
