Figure 4

(A) Boxplot showing the electron density measured in a gray scale of small (< 200 nm at maximum diameter) and large (> 200 nm at maximum diameter) seminal extracellular vesicles (sEVs). Higher values in the gray scale indicate lower electron density. Boxes enclose the 25th and 75th percentiles, the whiskers extend to the 5th and 95th percentiles and the line represent the median. ****P < 0.0001. (B) Representative cryo-electron microscopy images illustrating differences in electron density among small and large sEVs. Yellow arrows highlight content with filamentous or filiform structures.