Figure 5

(A) Representative cryo-electron microscopy (cryo-EM) images showing the structural complexity of porcine seminal plasma extracellular vesicles (sEVs). Most sEVs showed simple structures and only a few had one or more small EVs within them (see sEV highlighted with a star in the left micrograph) or were connected to each other either by membrane fusion or by channels connecting the lumens (see the sEV highlighted with a triangle in the right micrograph). (B) Representative cryo-EM micrographs showing sEVs with a peripheral coronal layer surrounding the EV membrane. The sEVs with a visible peripheral coronal layer are highlighted by yellow arrows.