Table 1 CCD for optimization of the immobilization process.
Trial | Independent variable | Recovery of immobilized enzyme (%) | Observed immobilization yield (%) | Predicted immobilization yield (%) | Residual | |
|---|---|---|---|---|---|---|
X1 Time of loading (h) | X2 Loading unit (U/g gel bead) | |||||
1 | 18 (−1) | 2.5 (−1) | 0.766 | 0.55 | 0.513185 | 0.036815 |
2 | 18 (−1) | 3.5 (+ 1) | 0.551 | 0.33 | 0.271096 | 0.058904 |
3 | 26 (+ 1) | 2.5 (−1) | 0.92 | 0.882 | 0.961569 | -0.07957 |
4 | 26 (+ 1) | 3.5 (+ 1) | 0.640 | 0.349 | 0.40644 | -0.05744 |
5 | 14 (−2) | 3 (0) | 0.532 | 0.215 | 0.267832 | -0.05283 |
6 | 30 (+ 2) | 3 (0) | 0.981 | 0.915 | 0.85156 | 0.06344 |
7 | 22 (0) | 2 (−2) | 0.995 | 0.972 | 0.955778 | 0.016222 |
8 | 22 (0) | 4 (+ 2) | 0.441 | 0.153 | 0.15856 | -0.00556 |
9 | 22 (0) | 3 (0) | 0.723 | 0.53 | 0.517712 | 0.012288 |
10 | 22 (0) | 3 (0) | 0.723 | 0.53 | 0.517712 | 0.012288 |
11 | 22 (0) | 3 (0) | 0.70 | 0.5 | 0.517712 | -0.01771 |
12 | 22 (0) | 3 (0) | 0.72 | 0.52 | 0.517712 | 0.002288 |
13 | 22 (0) | 3 (0) | 0.723 | 0.53 | 0.517712 | 0.012288 |
