Figure 2
MSC-EVs suppress ADAM17 to mitigate renal fibrosis. (A) Dual immunofluorescent staining of ADAM17 (red) and α-SMA (green) in the kidneys of control rats and UUO rats. Scale bars, 100 μm. (B) Immunofluorescent staining images of ADAM17 (red) and α-SMA (green) proteins in HK-2 cells treated with PBS or TGF-β. Scale bars, 50 μm. (C) Immunohistochemical staining of ADAM17 in kidney slices from UUO rats. Scale bars, 100 μm. (D) Western blot analysis of ADAM17, α-SMA, and Collagen I protein expression in the kidneys of control rats and UUO rats (n = 3). (E) Western blot analysis of ADAM17, α-SMA, and Collagen I protein expression in control and TGF-β-stimulated HK-2 cells (n = 3). All data are presented as means ± SEM. ***P < 0.001. Original blots are presented in Supplementary Fig. S8.
