Figure 2 | Scientific Reports

Figure 2

From: Ancestral lineages of dietary exposure to an endocrine disrupting chemical drive distinct forms of transgenerational subfertility in an insect model

Figure 2

The viability, DNA fragmentation and protamine deficiency of spermatozoa from parent, F1 and F2 generation males, assessed via flow cytometry (n = 10 replicates, each replicate consisting of sperm from 10 to 15 males, per treatment group). Parameters presented include the percentage of viable spermatozoa as represented by the % of cells negative for PI; percentage of sperm DNA fragmentation, considered as the ration of red to green fluorescence after staining with acridine orange and percentage of spermatozoa with protamine deficiency, considered as the population of spermatozoa with higher CMA3 fluorescence. Values presented are the mean ± SEM. Different superscript represents statistical differences (P < 0.05). Refer to Legend below: .

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