Figure 4 | Scientific Reports

Figure 4

From: Ancestral lineages of dietary exposure to an endocrine disrupting chemical drive distinct forms of transgenerational subfertility in an insect model

Figure 4

Sperm mitochondrial superoxide production and mitochondrial membrane potential in spermatozoa from parent, F1 and F2 generation males, assessed via flow cytometry (n = 10 replicates, each replicate consisting of sperm from 10 to 15 males, per treatment group). Parameters presented include percentage of viable spermatozoa with high mitochondrial superoxide production, considered as the Mitosox positive/Syber Green negative population and the percentage of spermatozoa with high mitochondrial membrane potential, represented by the population exhibiting a shift toward increased JC-10 fluorescence. Values presented are the mean ± SEM. Different superscript represents statistical differences (P < 0.05). Refer to Legend below: .

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