Figure 2

Amplicon distribution of the multiplex PCR after sequencing. The multiplex PCR was performed with an equimolar concentration (0.2 µM of each primer, n = 4) and with adapted primer concentrations off-chip (n = 12) and on-chip (n = 12). The used adapted primer concentration for each amplicon is from top down as follows: 0.6 µM, 0.4 µM, 0.6 µM, 0.2 µM, 0.6 µM, 0.2 µM.