Figure 3

Implementation of a magnetic bead based DNA purification process on the Vivalytic LoC cartridge. A 280 bp amplicon was purified with SPRIselect beads in different ways, quantified with the Qubit 1X dsDNA HS kit, and set to an off-chip reference (ref.). The reference (ref.) was processed according to the manufacturer's manual. (a) Off-chip testing (ref. n = 18) of microfluidic compatible elution buffer (EB, n = 5) and wash buffer (WB, n = 5). (b) Purification efficiency of dried magnetic beads after different storage times (n = 5, ref. n = 15). (c) Purification efficiency of liquid and dried magnetic beads resuspended either with chamber or peristaltic pumping on the Vivalytic cartridge. The beads were either applied in binding buffer (liquid: chamber pumping n = 5, peristaltic pump n = 4) or dried in the PCR chamber (dried: chamber pump n = 3, peristaltic pump n = 3, ref. n = 14). Error bars display standard deviation; x = median.