Figure 4
Quantity and quality control of on-chip prepared libraries to manually (off-chip) processed ones. (a) Stage I prepared libraries from targeted enrichment PCR until fully processed ligation (n = 4). Libraries were finished off-chip with 10 cycles in the index PCR. (b) Ligated DNA was normalized to one concentration and on-chip purified, amplified via index PCR (15 cycles), and again purified on cartridge (off-chip n = 4, on-chip n = 6). (c) Whole workflow (Stage I + II) with 10 ng gDNA input and 15 cycles in the index PCR (n = 4). Dashed lines indicate the minimum DNA library amount for sequencing of 4 nM for 25 μl of an average library size of 270 bp. Quantity measurements were performed with the Qubit 1X dsDNA HS kit. Quality controls were performed with the HS NGS Fragment Kit (digital blot images are cropped). Error bars display standard deviation.
