Figure 7 | Scientific Reports

Figure 7

From: Automation of customizable library preparation for next-generation sequencing into an open microfluidic platform

Figure 7

DNA quantification on the Vivalytic LoC cartridge with an automated dilution system for highly concentrated samples. (a) Samples were diluted automatically with Qubit 1X dsDNA high sensitivity working solution by mixing filled chambers on-chip (n = 3). After mixing, a picture was taken (excitation/emission 469 nm/ 525 nm) and diluted again. (b) Concentration determination on-chip during different dilution steps of four samples in comparison with the Qubit 4 Fluorometer as a reference. The relative deviations from the reference are given numerically. (blue labels): optimal dilution steps for quantification corresponding to the actual input concentration. Error bars display standard deviation.

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