Figure 1
From: Gnao1 is a molecular switch that regulates the Rho signaling pathway in differentiating neurons
RNA sequencing for Gαo-depleted Neuro2a cells. (a) Box-dot plot shows the relative expression of Gnao1 mRNA to actin-β (Actb) in Neuro 2a cells after treatment with siRNA against Gnao1 (siGnao1) or control siRNA. P = 0.100 (n = 3 each group; Wilcoxon’s rank-sum test). (b) Volcano plots for genes detected by RNA sequencing. Green lines indicate the cut-off levels: log2 (Fold Change) > 1 or < − 1; p < 0.05 (− log10 [p-value] > 1.301). Among the 1891 genes with a significant increase (red) or decrease (blue) in expression, the top 10 genes with high—log10 (p-values) were annotated. (c) Heat map clustering of rMATS data for the 2948 transcripts differentially expressed in siGnao1 and control siRNA-treated Neuro2a cells. The color gradient indicates a Z score > 0 (red: high) and < 0 (blue: low) for the expression of each transcript. (d) A Reactome pathway analysis on rMATS data. Bar plots represent p-values for over-representation (blue), the number (white) and percentage (%, orange) of genes annotated in each category. Red circles indicate the categories associated with Rho GTPase signaling. (e) GSEA for RhoA and RhoB GTPase cycle pathways in the Reactome. The expression of genes in these categories did not deviate from either the upregulated or downregulated side.
