Figure 6
From: Gnao1 is a molecular switch that regulates the Rho signaling pathway in differentiating neurons
Restoring the growth and differentiation of patient-derived organoids by Rho kinase inhibitor. (a) Phase-contrast images of patient (PT)-derived neuro-spheroids (arrows) after treatment with 50, 100 and 250 μM Y27632. Microscopic images were captured at the indicated time points (days 1–15). (b) Aria size of spheroids (μm2) on the indicated days of treatment. Box-dot plots are line-connected with the mean values of spheroid size at indicated days of differentiation. **p < 0.01, ***p < 0.001 (n = 6, Steel–Dwass’ multiple comparison [50 μM vs. 100 μM] and [50 μM vs. 250 μM]). Six independent spheroids were used for this analysis. (c) Immunofluorescence images of PT organoids treated with 50, 100, and 250 μM Y27632. Organoids were cryo-sectioned 15 days after treatment with Y27632. The dashed lines indicate the VZ region. Arrowheads indicate that round-shaped VZ structures were recovered after treatment with 100 μM Y27632. (d) NeuN/FoxG1 ratio in the VZ of PT organoids treated with 50, 100, and 250 μM Y27632. Small dots represent the value in each ROI (n = 1,106 [50 μM], 1,357 [100 μM] and 1438 [250 μM]) ***p < 0.001 (Kruskal–Wallis test). Three independent organoids were analyzed.
