Figure 6

TEM. Silver enhanced C-peptide nanogold immunostaining: (A) Pancreatic islet cell contains labeled c-peptide (black spots,arrows) in the cytoplasm. (B) Naïve MSC negative for C-peptide. (C) Differentiated MSC contains labeled C-peptide (blackspots,arrows) in the cytoplasm. (D) Naïve MSC cocultured with uneducatedEVs is negative for C-peptide. (E) Naïve MSC cocultured with educated EVsshows labeled C-peptidein the cytoplasm (black spots,arrows). Mitochondria (mito), rough endoplasmic reticulum (RER). To visualize the silver enhanced nanogold particles the TEM samples were not contrasted with osmium, thus the ultrastructural features of cells are not well visible.