Figure 7
The curve in the figure illustrates the dose–response relationship between dietary choline level and intestinal steatosis, as previously published by Hansen et al.3. The dotted lines illustrate the strategy used for estimation of effect on choline requirement in the present study. The increase in rapeseed oil from 0 to 24% increased the score by 1.7 units (orange line) in the large fish. The figure indicates that this shift corresponds to a shift in choline requirement of about 650 mg/kg. The shift in the small fish was 1.2, corresponding to a shift of 450 mg/kg (not illustrated). The difference between the two fish sizes, when fed the lowest rapeseed oil level, was 0.8 units (blue line), i.e. lower in the small than the large fish, corresponding to a difference in choline requirement of about 250 mg/kg. At the highest rapeseed oil level, the difference was 0.3, corresponding to a difference in choline requirement of about 100 mg/kg (not illustrated). As there were great differences in the experimental conditions between Hansen et al.3 experiment and the present, these estimates should be taken as indications of magnitude of effects rather than accurate estimates.
