Figure. 8

Astragaloside IV protected PC12 cells against apoptosis by inhibiting the p-GRK2 signaling pathway in vitro. The related protein levels of p-GRK2 signaling pathway (A) and apoptosis (B) were determined by Western blot. Representative blots and bar graphs from four groups were shown. Images were cropped, and full-length blots were presented in Supplementary Fig. 8. C Representative double immunofluorescence staining. The data are represented as the mean ± SEM. **P < 0.01, control group vs. Astragaloside IV group, ns means no statistical significance between the two groups. The error bars represent the SEM. Statistical analysis was performed using an unpaired, two-tailed Student’s t-test.