Figure 3
From: Splicing is dynamically regulated during limb development
Several differentially spliced genes are also differentially expressed. Volcano plots of the differentially expressed genes between the bud and paddle stages in (A) mouse and (B) opossum. Significantly downregulated genes are shown in green and significantly upregulated genes are shown in purple. Significance was determined by a − log10(FDR) > 1.3 (horizontal line) and either a logFC > 0.6 or a logFC < − 0.6 (vertical lines). (C, D) Venn diagrams showing the relationship between differentially expressed genes (blue), genes that are differentially spliced (orange), and genes exhibiting both differential expression and splicing (white) in the ridge to bud stage (left), and bud to paddle stage (right) in (C) mouse and (D) opossum. The percentage of differentially spliced genes that are also differentially expressed are shown below. Only genes used in both the differential gene expression and differential splicing analyses were kept. Ridge-Bud differential gene expression is shown in supplementary Fig. 4.
