Figure 5

KmTx2 pore-forming activity is influenced by the lipid composition of the bilayer. a. Single channel traces of KmTx 2 incorporated in lipid bilayers made from different types of lipids (phosphoethanolamine: phospho-l-serine: phosphocholine and tested lipid; 4.7:2.8:1.9:0.6 ratios). b. KmTx2 channel mean current amplitude (pA; black squares) plotted as a function of the membrane potential (mV) for the different tested lipids. Conductance was determined by linear regression (blue line) of the current values from -100 to 0 mV, in asymmetrical solutions (100/20 mM KCl cis/trans). Data are shown as means ± S.E.M. of 2–7 measurements.