Table 3 Stability assessment parameters of the optimal formula (F2) following storage at different temperature conditions (see Table 2 for F2 composition).

From: Quality by design approach of apocynin loaded clove oil based nanostructured lipid carrier as a prophylactic regimen in hemorrhagic cystitis in vitro and in vivo comprehensive study

Temp/humidity

Month

EE%

PS (nm)

PDI

ZP (mv)

Refrigerated conditions (4 ± 1 °C)

0

79.3 ± 0.9

214.8 ± 5.8

0.18 ± 0.01

37.5 ± 1.1

1

79.1 ± 0.5

215.3 ± 1.3

0.18 ± 0.05

37.6 ± 1.1

2

78.5 ± 0.3

215.7 ± 1.1

0.18 ± 0.07

37.6 ± 0.4

3

78.3 ± 0.1

216.1 ± 0.9

0.18 ± 0.08

37.6 ± 0.5

Ambient conditions (25 °C ± 2 °C/60% ± 5% RH)

0

79.3 ± 0.9

214.8 ± 5.8

0.18 ± 0.01

37.5 ± 1.1

1

75.3 ± 1.3*

223.2 ± 2.1*

0.22 ± 0.07*

29.8 ± 1.2*

2

70.1 ± 1.2*

233.6 ± 3.2*

0.34 ± 0.05*

25.3 ± 1.1*

3

63.9 ± 1.6*

245.3 ± 2.41*

0.36 ± 0.06*

23.9 ± 1.5*

  1. Each value represents the mean ± SD (n = 3).
  2. PS particle size (nm), PDI polydispersity index, ZP zeta potential (mv), EE% entrapment efficiency (%).
  3. *Significant at p < 0.05 monthly vs. initial.
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