Table 1 Baseline data for dogs in each of the cohorts included in the study.
Group | Age (median [range]) | Sex | Breed | Total urine creatinine of sample volume from which EVs were extracted (median [range]) | Small RNA content of 500uL of EV fraction (median [range]) | miRNA content of 500uL of EV fraction (median [range]) |
|---|---|---|---|---|---|---|
Sequencing UC (n = 12) | 10 [8–14] years, 1 unknown | 1 male entire 5 male neutered 6 female neutered | 1 each of Basset de Bretagne, Staffordshire Bull Terrier, Jack Russell Terrier, Schnauzer, Scottish Terrier, Labrador, Polish Lowland Sheepdog, Pug, Border Collie, Poodle, Weimaraner, Cross-breed | 17 [4–90] µmol | 17 [2–53] ng | 10 [1–18] ng |
Sequencing UTI (n = 5) | 9 [6–10] years, 1 unknown | 1 male neutered 1 male unknown status 2 female neutered 1 female entire | 2 Cocker Spaniel, 1 each of German Shepherd, Springer Spaniel and Boxer | 27 [2–106] µmol | 13 [6–80] ng | 2 [2–30] ng |
Sequencing HC (n = 6) | 5 [2–9] years | 4 male entire 1 male neutered 1 female neutered | 1 each of Finnish Spitz, Bichon Frise, Labrador, Dachshund, German Shepherd and Cross-breed | 59 [30–99] µmol | 12 [8–33] ng | 6 [3–16] ng |
Validation UC (n = 8) | 12 [11–13] years | 4 male neutered 3 female neutered 1 unknown | 2 Labradors, 2 Staffordshire Bull Terriers, 2 West Highland White Terriers, 1 each of Papillon and Beagle | 26 [13–30] µmol | 26 [17–65] ng | 12 [8–40] ng |
Validation UTI (n = 8) | 7 [0.25–10] years | 1 male entire 1 male neutered 1 female entire 4 female neutered 1 unknown | 2 Labrador, 1 each of Border Collie, Hungarian Viszla, Golden Retriever, Newfoundland, Staffordshire Bull Terrier and Pug | 19 [9–54] µmol | 24 [7–38] ng | 9 [3–21] ng |
Validation HC (n = 5) | 8 [2–12] years | 3 male neutered 2 female neutered | 2 Cocker spaniels, 1 each of Boxer, Border Terrier and Pug | 70 [26–82] µmol | 27 [10–33] ng | 11 [< 1–17] ng |
