Figure 3

RIP analysis confirms that lncTCF7 interacts with SND1. (A) Western blot analysis demonstrates successful IP of SND1. (B) Recovery of lncTCF7 and GAPDH RNA, assessed by RT-qPCR following RIP of SND1 versus an IgG control in U251 cells. The graph depicts the average recovery ± SEM (two-tailed ratio t-test, n = 4). (C) Recovery of lncTCF7 and GAPDH RNA, assessed by RT-qPCR following RIP of SND1 versus an IgG control in U87 cells. The graph depicts the average recovery ± SEM (two-tailed ratio t-test, n = 3). *P-value < 0.05, **P-value < 0.01, ns implies not significant.