Figure 6

Corticosterone secretion from NR5A1-ADSCs was increased by culture in collagen and laminin-coated dish. (A) ADSCs were inoculated with adeno-NR5A1, cultured overnight, plated in type IV collagen, laminin, or type IV collagen and laminin-coated plate, and cultured for 6 days. (B) The culture medium was collected, and the corticosterone and testosterone levels were measured using ELISA. Statistical analysis was performed using a one-way ANOVA (*p < 0.05, ns; not significant). ADSCs, adipose-derived stem cells; ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; NR5A1, nuclear receptor subfamily 5 group A member 1.