Figure 5
Reduced expression of cyp4d14, cyp4d8, and cpr66D affects D. melanogaster susceptibility to insecticides. (a) Relative expression of cyp4d14 in wild-type (w1118) and cyp4d14 mutant fly lines. Each point represents a biological replicate of 10 female bodies (n = 4), and error bars indicate ± SEM. Asterisks denote significant p-values as determined by one-sample t and Wilcoxon Test compared to a hypothetical mean of 1: * p < 0.05; **** p < 0.0001. (b, c) Mortality of w1118 (grey) and cyp4d14 (green) flies when exposed to sublethal concentrations of (b) zeta-cypermethrin and (c) spinosad. Each point represents a biological replicate of 5 males and 5 females (n = 5), and error bars indicate ± SEM. Asterisks denote significant p-values as determined by Two-way ANOVA followed by Holm-Sidak’s multiple comparisons test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Non-significant comparisons are denoted as “ns”. (d) Relative expression of cyp4d8 in wild-type (yw) and cyp4d8 mutant fly lines. (e, f) Mortality of yw (black) and cyp4d8 (red) mutant flies when exposed to sublethal concentrations of (e) zeta-cypermethrin or (f) spinosad. (g) Relative expression of cuticular protein (cpr) 66D in wild-type (w1118) or cpr66D mutant fly lines. (h, i) Mortality of w1118 (grey) or cpr66D (orange) flies when exposed to sublethal concentrations of (h) zeta-cypermethrin or (i) spinosad.
