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Figure 1

From: A serum-free culture medium production system by co-culture combining growth factor-secreting cells and l-lactate-assimilating cyanobacteria for sustainable cultured meat production

Figure 1

Evaluation of C2C12 cell proliferation following the addition of RL34 cell culture medium: (a–e) or 50:50 v/v% of DMEM-diluted RL34 cell culture medium: (f–j). (a,f) Fold change of C2C12 cell density relative to the initial cell density (a: 4.5 ± 0.70 × 104 cells/well; f: 4.8 ± 0.84 × 104 cells/well in 12-well plates) after culture with 1 mL of DMEM or serum-free RL34 medium for 2 days (a: n = 4; f: n = 3). The darker color of columns indicates higher seeding density of RL34 cells; 0.25 × 106 (light pink), 1.0 × 106 (pink), and 4.0 × 106 (crimson) cells/well in 6-well plate. (b–e), (g–j) Time-dependent changes in (b,g) glucose; (c,h) lactate; (d,i) ammonium; (e,j) LDH content of RL34 culture medium and 50:50 v/v% of DMEM-diluted RL34 cell culture medium (4.0 × 106 cells/well in 6-well plate, (b–e): n = 4, (g–j): n = 3). Data represent the mean ± SD; ***p < 0.001; **p < 0.01; *p < 0.05.

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