Figure 2
Comparing RL34 monoculture vs. RL34 and KC0110 strain transwell co-culture. The gray columns stand for RL34 monoculture (seeding density of 4.0 × 106 cells/well in 6-well plate for 3 days), and the green columns stand for RL34 and KC0110 strain transwell co-culture. The darker the green color of the column stands for the higher seeding density of KC0110; optical density at 750 nm (OD750) 1.0 (light green), 2.0 (green), 4.0 (dark green) cultured with 4.0 × 106 RL34 cells/well in 6-well plate for 3 days. (a) Fold change of RL34 cell density relative to the initial cell density (4.5 ± 0.50 × 106 cells/well in 6-well plates) co-cultured with KC0110 for 3 days (n = 4). (b) Change of OD750 to the initial cell density (Low: 0.97 ± 0.058; Mid: 2.0 ± 0.017; High: 4.0 ± 0.015) of KC0110 co-cultured with RL34 for 3 days (n = 3). (c–g) Metabolite content of co-culture medium (seeding density of 4.0 × 106 RL34 cells/well with OD750 2.0 KC0110 in 6-well plate for 3 days) compared to monoculture medium (seeding density of 4.0 × 106 RL34 cells/well without KC0110 in the 6-well plate for 3 days); (c) lactate; (d) ammonium; (e) glucose; (f) pyruvate and (g) LDH (n = 6). (h) 20 amino acids composition (n = 5); (i) total amount of 20 amino acids (n = 5). Data represent the mean ± SD; ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant.
