Figure 3 | Scientific Reports

Figure 3

From: A serum-free culture medium production system by co-culture combining growth factor-secreting cells and l-lactate-assimilating cyanobacteria for sustainable cultured meat production

Figure 3

Evaluation of the proliferation of C2C12 cells in RL34 and KC0110 co-culture medium (sup.). (a) Fold change of C2C12 cell density relative to the initial cell density (4.1 ± 0.62 × 104 cells/well in 12-well plates) following culture in different media (1 mL per well, respectively) for 2 days (n = 3); DMEM (white); RL34 monoculture medium (gray, seeding density of 4.0 × 106 cells/well in 6-well plate for 3 days); RL34 and KC0110 co-culture medium (light green, OD750 1.0; green, OD750 2.0; dark green, OD750 4.0 KC0110 with 4.0 × 106 RL34 cells/well in 6-well plate for 3 days). **p < 0.01; ns not significant. (b) Immunofluorescence staining for phalloidin (red) together with nucleus (blue) and MyoD (green) in C2C12 cells cultured in DMEM, RL34 monoculture medium, or RL34 and KC0110 (OD750 2.0) co-culture medium (scale bar: 200 µm). Data represent the mean ± SD; **p < 0.01; ns not significant.

Back to article page