Figure 7 | Scientific Reports

Figure 7

From: High glucose- or AGE-induced oxidative stress inhibits hippocampal neuronal mitophagy through the Keap1–Nrf2–PHB2 pathway in diabetic encephalopathy

Figure 7

Effects of PHB2 upregulation on the mitochondrial structural damage and mitophagy inhibition in the hippocampus of DE mice. (a) Representative TEM images of the ultrastructure of mitochondria in the hippocampal neurons of indicated groups (scale bar = 0.5 μm). (b, c) Representative images of Class I–III mitochondria (b) and their quantitative distribution (c) in the hippocampal neurons of the indicated groups. (d, e) Representative images of mitophagy (d) and the quantitative graphs of the number density of mitophagy (e) in the hippocampal neurons of the indicated groups; ***P < 0.001 vs CON group and †P < 0.05 and ††P < 0.01 vs DE group (scale bar = 0.5 μm; n = 5). (f) Representative immunofluorescence images of TOM20 (green) and LAMP2 (red) in the DG region of the hippocampus of the indicated groups (scale bar = 50 μm). (g–j) Representative WB images (g) and quantitative analysis of LC3II/LC3I (h), Beclin 1 (i), and TOM20 (j) in the hippocampus of the indicated groups; *P < 0.05 and **P < 0.01 vs CON group and †P < 0.05 vs DE group. The values are shown as the means ± SD, n = 3.

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