Fig. 4
Evaluation of lktA gene variants and lktA phylogeny. (a) Alignment of the predicted CDSs of the lkt region of F1260 of Clade B and the lktA region of F1291 of Clade A. Along F1291 two loci were identified as lktA-like genes (Mutated lktA 1 and Mutated lktA 2). (b) Pairwise nucleotide sequence alignment of lktA F1260 and ORF1 of lktA F1291. The deletion of two nucleotides in position 3918 and 3919, and the downstream TGA stop codon are displayed in the second line of the alignment corresponding to ORF1 of lktA F1291. (c) Phylogenetic tree obtained from the multiple sequence alignment of lktA nucleotide sequences. The length of lktA gene, or the full region where lktA ORF1 and ORF2 are predicted, were considered. Highlighted in green those genomes presenting the deletion in the lktA region that results in two predicted ORFs. The bootstrap values are reported along the phylogeny and the branches are colored according to them.
