Fig. 6
From: The Kv4 potassium channel modulator NS5806 attenuates cardiac hypertrophy in vivo and in vitro
Effects of NS5806 on ET-1-induced hypertrophy in NRVMs. (A) A schematic diagram of the experimental tests. (B) Representative confocal images of control, NS5806 (3 μM) or ET-1 (100 nM), and different concentrations of NS5806 combined with ET-1 for 48 h. The nuclei of the cardiomyocytes were stained with Hoechst (blue), and the intracellular cytoskeleton F-actin was stained with phalloidin (red). (C) Quantitative analysis of the CSA of NRVMs (n ≥ 50 cells from 3 primary cell preparations in each group). (D–F) Quantification of the mRNA levels of the hypertrophic markers ANP, BNP and TGF-β. GAPDH served as an internal control. (n = 3 in each group). *P < 0.05, ** P < 0.01 versus Con. #P < 0.05, ##P < 0.01 versus ET-1.
