Table 3 Regression and validation parameters of the proposed HPLC method for the determination of pure DIP, HBB and AMP in bovine urine.

Parameters	Standards
Parameters	DIP	HBB	AMP
Range µg/mL	15–75	2.5–60	2.5–60
Slope	1.1178	0.7914	0.1939
intercept	− 11.357	0.2956	0.2237
Correlation coefficient	0.9999	0.9998	0.9999
Accuracy ^amean ± SD	100.30 ± 0.23	100.27 ± 0.41	99.46 ± 0.48
Precision (RSD%) ^bRepeatability ^cIntermediate precision	0.332 0.124	0.446 0.288	0.426 0.600
^dLLOQ µg/mL	15	2.5	2.5
^eLQC µg/mL	45	7.5	7.5
^fMQC µg/mL	55	25	25
^gHQC µg/mL	60	50	50

^aMean recovery % and standard deviation of four determinations of DIP (15,45,55 and 60 ug/mL), HBB and AMP (2.5, 7.5, 25and 50 µg/mL).
^bThe intraday (n = 4) relative standard deviation of concentrations (15,45,55,60 ug/mL) of DIP and (2.5,7.5, 25,50 ug/mL) of HBB and AMP repeated three times within the day.
^cThe interday (n = 4) relative standard deviation of concentrations (15,45,55,60 ug/mL) of DIP and (2.5,7.5, 25,50 ug/mL) of HBB and AMP repeated three times in three days.
^dLowest limit of quantitation.
^eLow-Quality Control.
^fMiddle-Quality Control.
^gHigh-Quality Control.

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