Fig. 2 | Scientific Reports

Fig. 2

From: Circular RNA HIPK3 mediates epithelial–mesenchymal transition of retinal pigment epithelial cells by sponging multiple microRNAs

Fig. 2

Silencing of circHIPK3 attenuates RPE-EMT process and inhibits cell migration. (A) Expression of circHIPK3 detected by qRT-PCR (normalized to 18S rRNA expression, n = 3). *P < 0.05. (B) Expression of EMT-related markers detected by qRT-PCR (normalized to GAPDH expression, n = 3). *P < 0.05. (C) Expression of EMT-related markers detected by WB (n = 3). The representative images were shown. (D) Analysis of band intensities in WB data (normalized to β-actin expression, n = 3). *P < 0.05. (E) Expression of EMT-related markers in RPE cells treated with TGF-β1 + si-Scr or TGF-β1 + si-circHIPK3 detected by immunofluorescence staining (n = 3). Scale bar, 50 μm. (F) Transwell migration assays in RPE cells treated with TGF-β1 + si-Scr or TGF-β1 + si-circHIPK3 (n = 5). Scale bar, 50 μm. (G) Analysis of migrated cell number in the Transwell migration assays (n = 5). *P < 0.05 versus Ctrl group.

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