Fig. 2
From: ANGPTL4 plays a paradoxical role in gastric cancer through the LGALS7 and Hedgehog pathways
The effects of ANGPTL4 on GC cell proliferation, apoptosis, migration, invasion and angiogenesis. (A) The expression of ANGPTL4 protein in multiple GC cell lines by western blot. (B) The expression of ANGPTL4 in ANGPTL4 KD-SNU5 cells, ANGPTL4 OE-MKN7 cells, and ANGPTL4 OE-AGS cells. The effects of ANGPTL4 on GC cell proliferation by (C) MTT analysis and (D) EdU analysis (a representative image is shown at ×200 magnification). (E) FCM analysis was used to confirm the apoptosis ability of ANGPTL4 KD-SNU5 cells, ANGPTL4 OE-MKN7 cells, and ANGPTL4 OE-AGS cells. Wound healing analysis (a representative image is displayed at 40× magnification) (F) and Transwell invasion assays (a representative image is displayed at 200× magnification) (G) were used to confirm the migration and invasion ability of ANGPTL4 KD-SNU5 cells, ANGPTL4 OE-MKN7 cells, and ANGPTL4 OE-AGS cells. (H) Chorioallantoic membrane assays and (I) tube formation assays were used to confirm the angiogenesis ability of ANGPTL4 KD-SNU5 cells, ANGPTL4 OE-MKN7 cells, and ANGPTL4 OE-AGS cells in vivo and in vitro, respectively (a representative image is displayed at ×100 magnification). The effect of ANGPTL4 on HUVEC migration and proliferation in the conditioned medium of ANGPTL4 KD/Vector-SNU5 cells, ANGPTL4 OE/Vector-MKN7 cells, and ANGPTL4 OE/Vector-AGS cells, as determined by a wound healing assay (a representative image is displayed at 40× magnification) (J) or an EdU analysis (a representative image is displayed at 200× magnification) (K). *p < 0.05, **p < 0.01, ***p < 0.001.
