Fig. 5
From: ANGPTL4 plays a paradoxical role in gastric cancer through the LGALS7 and Hedgehog pathways
LGALS7 plays a key role in GC cell migration, invasion and angiogenesis mediated by ANGPTL4. (A) The migration ability of MKN7 cells transfected with NC, vector, ANGPTL4 OE, or ANGPTL4 OE plus LGALS7 OE and the migration ability of AGS cells transfected with NC, vector, ANGPTL4 OE, or ANGPTL4 OE plus LGALS7 KD determined by wound healing analysis (a representative image is displayed at 40× magnification). (B) Transwell invasion analysis was used to confirm the invasion ability of MKN7, Vector-MKN7, ANGPTL4 OE-MKN7, ANGPTL4 OE plus Vector-MKN7, ANGPTL4 OE plus LGALS7 OE-MKN7, AGS, Vector-AGS, ANGPTL4 OE-AGS, ANGPTL4 OE plus Vector-AGS, and ANGPTL4 OE plus LGALS7 KD-AGS cells (a representative image is shown at ×200 magnification). (C) Tube formation analysis was used to confirm angiogenesis in MKN7, Vector-MKN7, ANGPTL4 OE-MKN7, ANGPTL4 OE plus Vector-MKN7, ANGPTL4 OE plus LGALS7 OE-MKN7, AGS, Vector-AGS, ANGPTL4 OE-AGS, ANGPTL4 OE plus Vector-AGS, and ANGPTL4 OE plus LGALS7 KD-AGS cells (a representative image is shown at ×100 magnification). The proliferation and migration ability of HUVECs cocultured in conditioned medium from MKN7 cells transfected with NC, vector, ANGPTL4 OE, or ANGPTL4 OE plus LGALS7 OE and the proliferation and migration ability of AGS cells transfected with NC, vector, ANGPTL4 OE, or ANGPTL4 OE plus LGALS7 KD determined by (D) EdU analysis (a representative image is displayed at ×200 magnification) and (E) wound healing assay (a representative image is displayed at ×40 magnification). ***p < 0.001.
