Fig. 3
The release of glutamate from U-87 MG is reduced by LbNOX and mitoLbNOX. (A) Schematic image for glutamate imaging. (B) The representative photographs for glutamate imaging from U-87 MG expressed with iGluSnFR, Glutamate sensor. Scale bar = 50 μm. Fluorescence photographs from U-87 MG before and after TFLLR puffing. (C) Traces of glutamate imaging from naive cells (left, n = 39), cells expressing LbNOX (center, n = 29), and mitoLbNOX (right, n = 18). Scales bars = 0.02 ΔF/F0, 20 s time. The black trace is the glutamate signal for individual cells, and the red one is the average value of the cells. The red triangle is the puffing of 500 μM TFLLR at 20 s. (D. The bar graph shows the ΔF/F0 peak in each condition. An individual dot is data from a single cell. Data are reported as mean with SEM. Data were analyzed by a Tukey’s multiple comparisons test. *P = 0.0408, ***P = 0.0008.
