Fig. 4
Analysis of motility, invasion, and proliferation in GBM expressing LbNOX and mitoLbNOX. (A) The representative photographs of a wound healing assay from U-87 MG naive cells (left), LbNOX expression cells (center), and mitoLbNOX expression cells (right). These photographs show the assay at 0 h (top) and 24 h (bottom) after the scratch with the cell-free region (yellow line). Scale bar = 500 μm. (B) The bar graph shows the percentage of healing in each condition (naive and LbNOX: n = 15, mitoLbNOX: n = 9). (C) The representative photographs for the invasion assay were obtained by staining U-87 MG with DAPI for each condition. Scale bar = 500 μm. (D) The percentage of invasion was presented as a bar graph in each condition (naive: n = 3, LbNOX and mitoLbNOX: n = 5). (E) The bar graph shows the normalized proliferation O.D values (naive, LbNOX, and mitoLbNOX: n = 16). (F) The representative photographs of LbNOX (top) and mitoLbNOX (bottom) in U-373 MG, with photographs of GFP and DIC. Scale bar = 20 μm. (G–K) Same as (A–E). However, J is displayed as the number of DAPI. All individual dots are data from a single well. All data are reported as mean with SEM. All data were analyzed by one-way ANOVA followed by a Tukey’s multiple comparisons test.
