Fig. 5

Immune cells infiltration after MI. (A,B) Representative flow cytometry analysis (A) and quantification (B) of neutrophils in the infarcted hearts on Day 3 post-MI (n = 6–10). (C,D) Representative flow cytometry analysis (C) and quantification (D) of the iNOS+ macrophages in the infarcted hearts on Day 3 post-MI (n = 6–10). (E,F) Representative immunofluorescence images (E) and quantification (F) of the iNOS+F4/80+ cells in the ischemic myocardium at day 3 after MI (n = 5–10). Scale bar, 100 μm. Error bars represent SD and significance was determined by one-way ANOVA test (***p < 0.001, and ns for not significant).