Fig. 3

Editing of the csr-1 gene. (a) The csr-1 gene with exons (dark grey) and introns (light grey) is shown with the gRNA binding positions indicated in yellow (gRNA-c1) and blue (gRNA-c2); introns in light grey, exons in dark grey. (b) gRNA sequences. (c) Examples of sequence alignments of csr-1 sequences from edited fungi with the wild type csr-1 sequence. Deletions are marked with a red and insertions with a purple line. Dots and green background indicate identical nucleotides, dashes indicate missing nucleotides, orange background indicates additional nucleotides.