Fig. 4

Editing of the naa-2 gene. (a) The naa-2 gene with exons (dark grey) and introns (light grey) is shown with the gRNA binding positions indicated in yellow (gRNA-n1) and blue (gRNA-n2). (b) gRNA sequences. (c) Examples of sequence alignments of naa-2 sequences from edited fungi with the wild type naa-2 sequence. Deletions are marked with a red and insertions with a purple line. Dots and green background indicate identical nucleotides, dashes indicate missing nucleotides, orange background indicates additional nucleotides.