Table 2 Percentage of analyzed colonies carrying a mutation in the naa-2 gene after editing using gRNA-n1/gRNA-n2 alone or in combination with gRNA-c2.
From: A user-friendly CRISPR/Cas9 system for mutagenesis of Neurospora crassa
gRNA | Alone (%) | In combination with gRNA-c2 (%) |
|---|---|---|
gRNA-n1 | 5.26 | 55.5 |
gRNA-n2 | 0 | 16.7 |