Table 2 Percentage of analyzed colonies carrying a mutation in the naa-2 gene after editing using gRNA-n1/gRNA-n2 alone or in combination with gRNA-c2.

From: A user-friendly CRISPR/Cas9 system for mutagenesis of Neurospora crassa

gRNA

Alone (%)

In combination with gRNA-c2 (%)

gRNA-n1

5.26

55.5

gRNA-n2

0

16.7