Fig. 8
Validation of the differential expression of BCL10, CDKN2A, DAPK1, PYGM and TIMP1 in CRC cells and tissues. (A–E) mRNA expression levels of key genes in different cell lines (NCM460, HCT116, SW480 and RKO) were measured by qRT-PCR. The results were normalized to the reference gene GAPDH. Data are shown as means ± SDs, and two-tailed unpaired t tests were used for statistical analysis of each marker; n = 4 independent experiments. (F–J) The expression levels of key genes in CRC tissues (n = 30) and adjacent normal tissues (n = 30). (K–T) Analysis of the overall survival (OS) and disease-specific survival (DSS) of the key genes. (*P < 0.05, **P < 0.01, ***P < 0.001).
