Fig. 4

Minocycline attenuated LPS-induced H9c2 cell injury. (A) Chemical structure of minocycline. (B) Cells were treated with 0–160 μM Min for 24 h, and cell viability was detected by the CCK-8 assay. (C) LDH level was measured in Min-treated H9c2 cells. (D) Min improved cell viability in LPS-treated H9c2 cells. (E) Min decreased LDH release in LPS-treated H9c2 cells. Data were presented as mean ± SD; n = 3; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. control group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. LPS group.