Fig. 3

Knockdown of SLC4A4 inhibited PCa invasion and migration. (A, B) PCa cells were transfected with control shRNA or SLC4A4 shRNA and subjected to matrigel invasion and migration assays. Cells were seeded into the upper chambers, and after 24 h, cells were examined under a light microscope at 20 × magnification. The results are shown as the mean number of invasive and migrated cells, which were obtained by counting cells in five randomly selected fields. The results are presented as means ± standard error of three independent experiments, with a significant decease observed in the SLC4A4 knockdown group compared to the control (**p < 0.05).