Fig. 6
SLC4A4 knockdown aberrantly regulates different gene profiles in PCa cells (A) The intensities of protein bands detected in western blotting were measured using imageJ (NIH, Bethesda, MD, USA), OriginPro 2021 software (OriginLab Corporation, Northampton, MA, USA) was used to perform hierarchical clustering and heatmap analysis of protein expression/activation in PCa cells either control shRNA (shCON) or SLC4A4 shRNA (shSLC4A4). This analysis identified the coupling relationships that reveal the regulatory pathways driving cell proliferation. (B) A heatmap showing top 40 up‐ and downregulated genes after SLC4A4 knockdown in DU145 cells. (C) Volcano plot showing distribution of 1157 differentially expressed genes upon SLC4A4 knockdown in DU145 cells. Log2 fold change is represented in x‐axis, while y axis represents –log10 P value. Upregulated and downregulated genes are marked with dots (D) qPCR validation of the RNA Sequencing data in DU-145 cells (E) Gene Ontology (GO) biological process enrichment analysis of the genes differentially expressed upon SLC4A4 knockdown in Prostate Cancer (PCa) cells. (F) Members of the SLC4 transporter family that were altered after SLC4A4 knockdown in PCa cells.
