Fig. 5
Detection of total Ig, IgG and IgA in DBS (A) and DSS (B) obtained from 42 individuals during the autumn of 2023. For the DBS detection, antibodies were eluted first from ø1.2 mm cuts in 100 µl elution buffer, and then 1 µl of elute (100-fold dilution) was used for the assay. Whereas the detection of antibody in DSS, ø1.2 mm cuts were directly incubated with a buffer containing oligonucleotide-antigen probes. All 42 DBS and DSS samples were analyzed in the same 96-well plate. To compensate for differences in the amounts of material from the different sample types 5.64 (log250) was added to the final ∆Ct values for DBS, corresponding to the 50-fold less sample input from DBS over those in DSS. Lines in the middle of each group represent the average ∆Ct values. The corresponding of total and IgG and IgA levels for individual sample donors are indicated by grey lines.
