Fig. 2 | Scientific Reports

Fig. 2

From: Liver-specific Coxsackievirus and adenovirus receptor deletion develop metabolic dysfunction–associated fatty liver disease

Fig. 2

CAR interacts with a new partner, APOBEC3C. (A) FLAG-CAR was overexpressed and pulled down by anti-FLAG agarose to find an interaction partner. The pull-down protein band was sliced and subjected to mass spectrometry. A new interaction protein, APOBEC3C, was identified. (B) These two protein interactions were confirmed by co-immunoprecipitation after overexpression of FLAG-CAR and GFP-APOBEC3C. Total protein was extracted and subsequently pulled down by anti-FLAG agarose. Agarose binding protein was subjected to western blot analysis using anti-GFP antibodies. (C) The opposite experiment was also performed after APOBEC3C-FLAG overexpression. FLAG agarose pull-down precipitated intracellular proteins were subjected to western blot analysis using anti-CAR antibodies.

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