Fig. 3 | Scientific Reports

Fig. 3

From: Liver-specific Coxsackievirus and adenovirus receptor deletion develop metabolic dysfunction–associated fatty liver disease

Fig. 3

Transient CAR deletion increases lipid transport regulatory protein and macrophage migration. (A,B) HepG2 cells were transfected with siCAR and scramble RNA as control (Con). Oleic acid treatment and Oil Red O staining were performed to determine lipid uptake levels in CAR deletions. At various time points (24, 48, 72, and 80 h) after siCAR delivery, cellular proteins were extracted and subjected to western blot analysis using anti-ApoB, -LDLR, -APOBEC3C, -CAR, and -GAPDH antibodies. (C) HepG2 cell lipid uptake was investigated by Oil red O staining after siRNA delivery. (D) Transwell chamber macrophage migration assay was performed after siCAR (for deletion) or mouse CAR (mCAR; for overexpression) transfection into bottom HepG2 cells. The upper chamber transwell passed macrophage was stained by Eosin (pink dot). Data are expressed as mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001.

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