Fig. 3
UC-MSC-CM inhibits Rho/MRTF/SRF signaling in HEFs. HEFs were treated with TGF-β1 (5 ng/mL) and cultured with or without UC-MSC-CM (24 h for RT-qPCR analysis, 48 h for western blotting). (A) RT-qPCR analysis of the relative mRNA expression of MRTFA, SRF, RHOA, ROCK1, ROCK2, and SRC. Data were normalized to GAPDH expression and expressed as relative values compared to the control (n = 3). (B) Representative western blots showing the protein expression of MRTF-A and SRF in the nuclear extracts with histone deacetylase (HDAC1) as a loading control, and RhoA in the cytoplasmic extracts with GAPDH as a loading control. (C) Quantitation of MRTF-A, SRF, and RhoA from western blot analyses (n = 4). Data are expressed as the mean ± SEM. ##P < .01 and ###P < 0.001 versus the control; *P < 0.05, **P < 0.01, and ***P < 0.001 versus TGF-β1 treatment only (ANOVA w/ Tukey). Original images of blots are presented in Supplementary Fig. S2.
