Fig. 5
UC-MSC-CM inhibits YAP/TAZ signaling in HEFs. HEFs were treated with TGF-β1 (5 ng/mL) and cultured with or without UC-MSC-CM (24 h for RT-qPCR analysis, 48 h for western blotting). (A) RT-qPCR analysis of the relative mRNA expression of YAP1 and WWTR1. Data were normalized to GAPDH expression and expressed as relative values compared to the control (n = 3). (B) Representative western blots showing the protein expression of YAP and TAZ in the nuclear extracts with histone deacetylase (HDAC1) as a loading control, and p-YAP in the cytoplasmic fraction with GAPDH as a loading control. (C) Quantitation of YAP, TAZ, and p-YAP from western blot analyses (n = 4 except for p-YAP (n = 6)). Data are expressed as the mean ± SEM. #P < 0.05, ##P < 0.01, and ###P < 0.001 versus the control; *P < 0.05, **P < 0.01, and ***P < 0.001 versus TGF-β1 treatment only (ANOVA w/ Tukey). Original images of blots are presented in Supplementary Fig. S4.
